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Prediction equations to estimate the demand of energy and crude protein for maintenance, gain and egg production for laying Japanese quails

Jordão Filho, José; Silva, José Humberto Vilar da; Costa, Fernando Guilherme Perazzo; Sakomura, Nilva Kazue; Silva, Cidinei Trajano; Chagas, Nalberlania Alves
Fonte: Sociedade Brasileira de Zootecnia Publicador: Sociedade Brasileira de Zootecnia
Tipo: Artigo de Revista Científica Formato: 2423-2430
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); The study was conducted to estimate requirements of energy and crude protein for maintenance, weight gain and egg production of Japanese quails in the period of 67 to 107 days of age. Two experiments were performed: one, to determine the requirements for maintenance of protein, and the other, for energy. Experiments were conducted in a completely randomized design with four dietary levels of offer and four replicates of six birds. The diets offered were: ad libitum (100%), 75%, 50%, and 25% consumption ad libitum (below maintenance). The methodology used to estimate the demand for maintenance was the comparative slaughter. For estimation of the requirement for weight gain, eight groups of 15 quails were reared separately, fed ad libitum and housed under 22 ºC controlled temperature. From these poultry, three groups were slaughtered at 5, 10, 15, 20, 25, 30, 35 and 40 days of the trial. The requirement for egg production was obtained by taking the ratio of energy content, egg protein, efficiency of energy use and protein for egg production. Prediction equations that estimate maintenance requirement...

Thermal studies on protein isolates of white lupin seeds (Lupinus albus)

Fontanari, Gustavo Guadagnucci; Martins, Jose Manuel; Kobelnik, Marcelo; Pastre, Ieda Aparecida; Gomes Areas, Jose Alfredo; Batistuti, Jose Paschoal; Fertonani, Fernando Luís
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica Formato: 141-148
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); This study used TG, DSC, and SDS-PAGE techniques to study protein isolates (PIs) in the powder form obtained from lupin seeds flour Lupinus albus. Different methods of preparing PIs were tested, resulting in final products that were different only in relation to the yield and protein content. The results of the protein analysis by SDS-PAGE showed that the same protein fractions were present in the lupin seeds and in the obtained PIs. This result shows that the process of extraction was not damaging to the composition of the original protein. on the other hand, the results of the thermal analysis (DSC and TG-DTG curves) obtained for the different PIs, led to the detection of changes in the protein conformation through the Delta H values, which in general decreased with increasing values of pH and ionic strength in the experimental conditions of extraction.

Expression of renin-angiotensin system signalling compounds in maternal protein-restricted rats: effect on renal sodium excretion and blood pressure

Mesquita, Flavia Fernandes; Rocha Gontijo, Jose Antonio; Boer, Patricia Aline
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica Formato: 380-388
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 05/543624; Methods. The pregnancy dams were divided in two groups: with normal protein diet (pups named NP) (17% protein) or low protein diet (pups LP) (6% protein) during all pregnancy.Results. The present data confirm a significant enhancement in arterial pressure in the LP group. Furthermore, the study showed a significantly decreased expression of RAS pathway protein and Ang II receptors in the kidney and an increased expression in the adrenal of LP rats. The detailed immunohistochemical analysis of RAS signalling proteins in the kidney confirm the immunoblotting results for both groups. The present investigation also showed a pronounced decrease in fractional urinary sodium excretion in maternal protein-restricted offspring, compared with the NP age-matched group. This occurred despite unchanged creatinine clearance.Conclusions. The current data led us to hypothesize that foetal undernutrition could be associated with decreased kidney expression of AT(R) resulting in the inability of renal tubules to handle the hydro-electrolyte balance...

Recovery of rat growth and lipid profiles in adult rats subjected to fetal protein malnutrition with a fructose-rich diet

Cambri, Lucieli T.; Ghezzi, Ana C.; Ribeiro, Carla; Dalia, Rodrigo A.; Rostom de Mello, Maria A.
Fonte: Pergamon-Elsevier B.V. Ltd Publicador: Pergamon-Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 156-162
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 08/53255-8; There is evidence suggesting an association between fructose consumption and the development of metabolic syndrome. In turn, protein malnutrition in utero is proposed to "program" the fetal tissues, making them more susceptible to nutritional associated disorders. To test this hypothesis, the present study was designed to analyze body growth and metabolic aspects of rats subjected to fetal protein malnutrition and subsequently fed a fructose-rich diet. Wistar rats were distributed into 4 groups: balanced (B) diet B diet offered the entire experimental period; balanced diet/fructose B diet until birth and fructose-rich diet (F-60% fructose) until adulthood; low-protein (L) diet/balanced L diet until birth and B diet until adulthood; low-protein diet/fructose (F) L diet until birth and F diet until adulthood. After nutritional recovery, there was a restoration of serum glucose, total protein, and albumin concentrations, which were reduced by fetal malnutrition, and a restoration of the liver glycogen and lipids contents...

Protein, methionine+cystine and lysine levels for Japanese quails during the production phase

Garcia, Edivaldo Antônio; Mendes, AA; Pizzolante, CC; Saldanha, ESPB; Moreira, J; Mori, C; Pavan, AC
Fonte: Fundação APINCO de Ciência e Tecnologia Avícolas Publicador: Fundação APINCO de Ciência e Tecnologia Avícolas
Tipo: Artigo de Revista Científica Formato: 11-18
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An experiment was conducted at Faculdade de Medicina Veterinária e Zootecnia/Unesp - Botucatu for 168 days to evaluate the effects of protein, Met + Cys and lysine diet levels on egg production and egg quality of laying Japanese quails. Quails with 42 days of age were reared in a completely randomized design. There were 1,944 quails distributed in four replicates of 27 birds per pen, according to a factorial 3x3x2 with three crude protein levels (16, 18 and 20% CP), three Met + Cys levels (0.700; 0.875 and 1.050%) and two lysine levels (1.100 and 1.375%). Birds fed diets with 18 and 20% CP had higher feed intake and egg production than those fed diets with 16% CP. There was significant interaction (p0.05) of the protein level on feed conversion per dozen eggs; however, improved feed conversion per egg mass was seen for birds fed diets with 20% CP compared to those fed diets with 16% and 18% CP. Protein and lipid percentage in the yolk increased when dietary protein level increased from 16 to 18%. Increasing Met + Cys from 0.700% to 0.875% reduced yolk protein percentage. Higher lipid percentage in the yolk was seen in eggs from quails fed diets with 1.050% Met + Cys, whereas 1.375% lysine in the diet of resulted in decreased egg production and egg mass...

Study of the proteins in the defatted flour and protein concentrate of baru nuts (Dipteryx alata Vog)

Guimarães, Rita de Cássia Avellaneda; Favaro, Simone Palma; Viana, Antonio Camilo Arguelho; Braga Neto, José Antônio; Neves, Valdir Augusto; Honer, Michael Robin
Fonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos Publicador: Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Tipo: Artigo de Revista Científica Formato: 464-470
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O baru (Dipteryx alata Vog.) é uma leguminosa abundante no Cerrado brasileiro, cuja castanha pode ser explorada através do uso sustentável para o aproveitamento das frações proteicas e lipídicas. Este trabalho teve como objetivo estudar as proteínas desta castanha, presentes na farinha desengordurada e no concentrado proteico, quanto as suas propriedades funcionais, ao perfil das frações proteicas e à digestibilidade in vitro. A farinha desengordurada com hexano foi submetida à extração no pH de maior solubilidade das proteínas, obtendo-se o concentrado proteico. O perfil eletroforético das frações proteicas foi avaliado em gel de SDS-PAGE. As propriedades funcionais indicaram a possibilidade de emprego em diversos alimentos, assim como a soja, conferindo capacidade de absorção de água, capacidade de absorção de óleo, propriedades emulsificantes e espumabilidade. As globulinas, seguidas das albuminas, são as frações majoritárias da farinha e do concentrado proteico, respectivamente. A digestibilidade foi superior no concentrado em relação à farinha desengordurada.; Baru (Dipteryx alata Vog.) is an abundant legume in the Brazilian Savanna. Its nuts can be exploited sustainably using its protein and lipid fractions. This study aimed to analyze the proteins of the nuts present in the defatted flour and protein concentrate in terms of their functional properties...

EFFECTS OF PROTEIN-DEFICIENCY AND NATURAL INTESTINAL INFECTION WITH GIARDIA-LAMBLIA ON JEJUNAL INTRAEPITHELIAL LYMPHOCYTES IN RATS OF DIFFERENT AGES

Rodrigues, MAM; Decarvalho, MFG; Montenegro, M. R.
Fonte: Associação Brasileira de Divulgação Científica (ABRADIC) Publicador: Associação Brasileira de Divulgação Científica (ABRADIC)
Tipo: Artigo de Revista Científica Formato: 1347-1353
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1. The interaction between experimental protein deprivation and natural intestinal infection by Giardia lamblia was studied in terms of its effects on the intraepithelial lymphocyte (IEL) population and morphology of the jejunal mucosa of rats of different ages.2. Young, adult and old male Wistar rats received a protein-deficient diet (2% casein) or a control diet (20% casein) for 42 days. Mucosal height and the number of lymphocytes located among 500 consecutive epithelial cells (EC) along the villi or crossing the basement membrane were determined in PAS-stained jejunal fragments.3. The number of IEL increased progressively with animal age, from 14 to 25 per 100 epithelial cells, with significant differences between age ranges. However, the number of IEL did not differ between control and protein-deficient rats in any of the age groups. The proportion of lymphocytes crossing the basement membrane was approximately two-fold greater in young (2.8/100 EC) and adult (5.8/100 EC) protein-deficient animals than in their respective controls (1.6 and 2.8/100 EC). The intensity of parasite colonization was moderate, from 3 to 5/100 EC and did not differ between groups. The pattern of morphologic changes of jejunal mucosa in protozoal infection did not differ between control and protein-deficient animals in any of the three age groups.4. We conclude that intestinal infection with Giardia lamblia probably stimulated the local immune response...

Effects of soy protein and resistance exercise on body composition and blood lipids in postmenopausal women

Maesta, Nailza; Nahas, Eliana A. P.; Nahas-Neto, Jorge; Orsatti, Fabio L.; Fernandes, Cesar E.; Traiman, Paulo; Burini, Roberto C.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 350-358
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Objective: To assess the effect of soy protein and progressive resistance training on body composition and lipids in postmenopausal women.Design: In a controlled trial, 46 postmenopausal women were randomized to one of four groups: 25 g of soy protein (SP, n = 10), 25 g of soy protein plus resistance exercise (SPE, n = 14), 25 g of maltodextrine (placebo) (PL, n = 11), or placebo plus resistance exercise (PLE, n = 11). Progressive resistance training was held three times a week for 16 weeks and included 8 exercises (3 series of 8-12 repetitions). At baseline and after 16 weeks, body mass index, waist circumference (WC), body fat, muscle mass and serum lipid levels were measured. To confirm isoflavone absorption, urinary concentrations were determined. The t-test of Student and ANOVA were used in the statistical analysis.Results: Subjects were classified as overweight and showed android fat distribution: Urinary isoflavone excretion indicated compliance to soy protein treatment. After 16 weeks of intervention, both SPE and PLE groups showed a significant increase of 1.3 kg in muscle mass and reduction in WC of -1.4 and -2.1 cm, respectively (p < 0.05). Significant decreases in the mean values of total cholesterol and LDL (-29.0 and -24.0 mg/dL...

EFFECTS OF INTRAUTERINE AND POSTNATAL PROTEIN-CALORIE MALNUTRITION ON METABOLIC ADAPTATIONS TO EXERCISE IN YOUNG-RATS

Demello, MAR
Fonte: Associação Brasileira de Divulgação Científica (ABRADIC) Publicador: Associação Brasileira de Divulgação Científica (ABRADIC)
Tipo: Artigo de Revista Científica Formato: 2461-2466
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The effect of intrauterine and postnatal protein-calorie malnutrition on the biochemical ability to perform exercise was investigated in young male rats. Malnourished rats were obtained by feeding dams a low-protein (6%) casein-based diet prepared in the laboratory during pregnancy and lactation. Control rats received an isocaloric diet containing 25% protein. The low-protein diet contained additional starch and glucose. At 45 days of age, malnourished rats showed lower body weight, serum protein, albumin and glucose levels, hematocrit values and heart glycogen content but higher circulating free fatty acids and gastrocnemius muscle glycogen than control rats. In response to exercise (50 min of swimming), control rats displayed lower heart, gastrocnemius and liver glycogen levels whereas malnourished rats showed low glycogen levels only in the gastrocnemius muscle. Both control and malnourished rats showed high serum glucose and free fatty acid levels after exercise. In conclusion, protein-calorie malnutrition improved muscle glycogen storage but this substrate was broken down to a greater extent in response to exercise. Malnourished rats were able to perform exercise maintaining high blood glucose levels, as observed in control rats...

Minimum blood lactate and muscle protein of rats during swimming exercise

Voltarelli, F. A.; Gobatto, C. A.; de Mello, M. A. R.
Fonte: Inst Sport Publicador: Inst Sport
Tipo: Artigo de Revista Científica Formato: 23-34
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Few studies dealing with effort intensity during swimming exercise in rats have been reported in the literature. Recently, with the use of the lactate minimum test (LMT), our group estimated the minimum blood lactate (MBL) of rats during swimming exercises. This information allowed accurate evaluation of the effort intensity developed by rats during swimming exercise. The present study was designed to evaluate the effects of swimming exercise sessions in below, equivalent and above intensities to MBL, on protein metabolism of rats. Adult (90 days) sedentary male Wistar rats were used in the present study. Mean values of MBL, in the present study, were obtained at blood concentration of 6.7 +/- 0.4 mmol/L with a load of 5% bw. The animals were sacrificed at rest (R) or immediately after a single swimming session (30 min) supporting loads below (3.5% bw), equivalent (5.0% bw) and high load (6.5% bw) to AT. Blood samples were collected each 5 min of exercise for lactate determination. Soleus muscle protein synthesis (amount of L-[C-14] fenil alanyn incorporation to protein) and breakdown (tyrosin release) rates were evaluated. Blood lactate concentrations (mmol/L) stabilized with the below (5.4 +/- 0.01) and equivalent (6.4 +/- 0.006) to MBL but increased...

Expression, purification and spectroscopic analysis of an HdrC: An iron-sulfur cluster-containing protein from Acidithiobacillus ferrooxidans

Ossa, D. M. H.; Oliveira, R. R.; Murakami, M. T.; Vicentini, R.; Costa-Filho, A. J.; Alexandrino, F.; Ottoboni, L. M. M.; Garcia, O.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 1335-1341
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Iron-sulfur cluster-containing proteins are present in all living organisms and are considered to be very ancient due to their ubiquity on the three domains of life, their importance in anaerobic metabolic pathways and energy conservation mechanisms. To date, there is little information regarding these proteins in Acidithiobacillus ferrooxidans, a bacterium involved in bioleaching processes. In this study are described the cloning, expression. purification and spectroscopic characterization of a new Fe-S cluster-containing protein, a putative HdrC homologue, from A. ferrooxidans strain LR. The oligomeric state of the protein was assessed by both dynamic light scattering and size-exclusion chromatography, demonstrating that it is present as a monomer in solution. Far-UV CD measurements revealed that this protein is extremely stable at acidic pHs, in which it assumes a different structure that exhibits a predominance of (x-helixes. In contrast, at basic pHs, from 9 to 12, the protein showed a spectral profile which is characteristic for proteins with high content of beta structure and random coil. Thermal unfolding studies demonstrated that this protein tolerates high temperatures at acidic conditions...

Role of different proteolytic pathways in degradation of muscle protein from streptozotocin-diabetic rats

Pepato, Maria Teresa; Migliorini, Renato H.; Goldberg, Alfred L.; Kettelhut, Isis C.
Tipo: Artigo de Revista Científica
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In vitro rates of overall proteolysis and the activities of four different proteolytic pathways (lysosomal, Ca2+ dependent, ATP dependent, and ATP independent), as well as rates of protein synthesis, were measured in soleus and extensor digitorum longus (EDL) muscles from streptozotocin- diabetic rats. In the acute phase (1-3 days) of diabetes, there was an increase in overall proteolysis that coincided with an increased activity of the Ca2+-dependent pathway in both soleus and EDL and of the ATP-dependent pathway in EDL. After longer periods (5-10 days) of diabetes, the overall rate of protein degradation decreased and reached values similar to or even lower than those of controls as a result of a reduction in the activities of Ca2+-dependent and ATP-dependent pathways. No change was detected at any time interval in the activity of the intralysosomal proteolytic system in muscles from diabetic animals. Rates of protein synthesis were already reduced 24 h after diabetes induction and decreased further thereafter. Insulin treatment restored to normal the activities of the proteolytic pathways and rates of protein synthesis.

Importance of early and continuous use of protein restriction on the progression of adriamycin nephropathy

Barretti, Pasqual; Soares, Vitor
Tipo: Artigo de Revista Científica Formato: 603-613
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Three experimental protocols were carried out with the aim of evaluating the role of protein restriction on the progression of the established adriamycin-induced nephropathy, and whether the protective effect of the diet persists after the diet is discontinued. The effect of a low protein diet (LPD) was studied for 6 weeks in protocol 1, 16 weeks in protocol 2 and for 28 weeks in protocol 3. In protocol 3, one group (LL) received LPD and another (NN) was given a normal protein diet (NPD). A third group (LN) received LPD for 16 weeks and then NPD for 12 weeks and a fourth group (NL) was fed NPD for 16 weeks and then LPD for 12 weeks. In protocol I the tubulo- interstitial index (TILl) of rats on LPD (Md = 2, P25 = 0.0; P75 = 3.5) after six weeks, was smaller than that of the animals on NPD (Md = 6.0; P25 = 3.0; P75 = 8.0; p < 0.05). In protocol 2, the group taking LPD presented an area of interstitial fibrosis (IF) (Md= 0.5%, P25 0.2%; P75 = 1.9%) smaller than that of the NPD group (Md = 6.8%; P25 = 5.2%; P75 = 7.1%; P < 0.05). No significant difference in the area of glomerulosclerosis (GSA) was observed between the animals on LPD (Md = 0.0%; P25 = 0.0%, P75 = 0.0%) and NPD (Md = 0.37%; P25 = 02% P75 = 1.25%; p > 0.05). In protocol 3...

Gestational protein restriction induces CA3 dendritic atrophy in dorsal hippocampal neurons but does not alter learning and memory performance in adult offspring

Lopes, A.; Torres, D. B.; Rodrigues, A. J.; Cerqueira, J. J.; Pêgo, J. M.; Sousa, N.; Gontijo, J. A R; Boer, P. A.
Tipo: Artigo de Revista Científica Formato: 151-156
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Studies have demonstrated that nutrient deficiency during pregnancy or in early postnatal life results in structural abnormalities in the offspring hippocampus and in cognitive impairment. In an attempt to analyze whether gestational protein restriction might induce learning and memory impairments associated with structural changes in the hippocampus, we carried out a detailed morphometric analysis of the hippocampus of male adult rats together with the behavioral characterization of these animals in the Morris water maze (MWM). Our results demonstrate that gestational protein restriction leads to a decrease in total basal dendritic length and in the number of intersections of CA3 pyramidal neurons whereas the cytoarchitecture of CA1 and dentate gyrus remained unchanged. Despite presenting significant structural rearrangements, we did not observe impairments in the MWM test. Considering the clear dissociation between the behavioral profile and the hippocampus neuronal changes, the functional significance of dendritic remodeling in fetal processing remains undisclosed. © 2012 ISDN.

Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Pinheiro, Daniela F.; Pinheiro, Patricia F.F.; Buratini, José; Castilho, Anthony C.S.; Lima, Paula F.; Trinca, Luiza A.; Vicentini-Paulino, Maria de Lourdes M.
Tipo: Artigo de Revista Científica Formato: 281-289
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Intrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later in life, an effect known as fetal programming. The present study evaluated mRNA abundance and immunolocalization of nutrient transporters as well as enterocytes proliferation in the proximal, median and distal segments of small intestine of rats born to protein-restricted dams. Pregnant rats were fed hypoproteic (6% protein) or control (17% protein) diets, and offspring rats were evaluated at 3 and 16 weeks of age. The presence of SGLT1 (sodium-glucose co-transporter 1), GLUT2 (glucose transporter 2), PEPT1 (peptide transporter 1) and the intestinal proliferation were evaluated by immunohistochemical techniques and the abundance of specific mRNA for SGLT1, GLUT2 and PEPT1 was assessed by the real-time PCR technique. Rats born to protein-restricted dams showed higher cell proliferation in all intestinal segments and higher gene expression of SGLT1 and PEPT1 in the duodenum. Moreover, in adult animals born to protein-restricted dams the immunoreactivity of SGLT1, GLUT2 and PEPT1in the duodenum was more intense than in control rats. Taken together, the results indicate that changes in the small intestine observed in adulthood can be programmed during the gestation. In addition...

High-fiber diets with reduced crude protein for commercial layers

Praes, M. F. F. M.; Junqueira, O. M.; Pereira, A. A.; Filardi, R. S.; Duarte, K. F.; Sgavioli, S.; Alva, J. C. R.; Domingues, C. H. F.
Fonte: Fundação APINCO de Ciência e Tecnologia Avícolas Publicador: Fundação APINCO de Ciência e Tecnologia Avícolas
Tipo: Artigo de Revista Científica Formato: 43-49
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This study aimed at evaluating diets containing different fiber sources and two crude protein levels on the performance, egg quality, and nitrogen metabolism of commercial layers. In total, 392 48-wk-old Isa Brown layers were distributed according to a completely randomized experimental design in a 3x2+1 (control) factorial arrangement, resulting in seven treatments with seven replicates of eight birds each. Treatments consisted of three fiber feedstuffs (cottonseed hulls, soybean hulls, and rice hulls) and two dietary crude protein levels (12% and 16%). Cottonseed hulls associated with the high crude protein level (16%) resulted in the worst feed conversion ratio per dozen eggs. Diets with 16% crude protein resulted in the highest feed intake, egg production, egg weight, egg mass values, and improved feed conversion ratio (kg eggs/kg feed). The dietary inclusion of soybean hulls determined low yolk pigmentation, and of rice hulls, low egg specific gravity. The 16% crude protein diet with rice hulls promoted the best feed conversion ratio. Hens fed the reference diet presented higher egg mass and better feed conversion ratio per kg eggs and per dozen eggs. Hens fed the diets with low crude protein level (12%) had reduced nitrogen excretion...

Ineraction Model Between HRSV G-Protein and Flavonoids

Souza, Fátima Pereira de; Sabbag, Mariana Pela; Araujo, Gabriela Campos de; Cravo, Haroldo lima Pimentel; Teixeira, Thiago Salen P; Gomes, Deriane Elias; Fadel, Valmir; Fossey, Marcelo Andrés
Tipo: Artigo de Revista Científica Formato: 12-19
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Background: Acute respiratory infections (ARI) are the leading cause of infant mortality in the world, and human respiratory syncytial virus (HRSV) is one of the main agents of ARI. One of the key targets of the adaptive host immune response is the RSV G-protein, which is responsible for attachment to the host cell. There is evidence that compounds such as flavonoids can inhibit viral infection in vitro. With this in mind, the main purpose of this study was to determine, using computational tools, the potential sites for interactions between G-protein and flavonoids. Results: Our study allowed the recognition of an hRSV G-protein model, as well as a model of the interaction with flavonoids. These models were composed, mainly, of ?-helix and random coil proteins. The docking process showed that molecular interactions are likely to occur. The flavonoid kaempferol-3-O-?-L-arabinopyranosil-(2 ? 1)-?-L-apiofuranoside-7-O-?-L-rhamnopyranoside was selected as a candidate inhibitor. The main forces of the interaction were hydrophobic, hydrogen and electrostatic. Conclusions: The model of G-protein is consistent with literature expectations, since it was mostly composed of random coils (highly glycosylated sites) and ?-helices (lipid regions)...

Analise das ações da dexametasona sobre a secreção de insulina, parametros bioquimicos e moleculares em ratos submetidos a restrição proteica; Analysis of dexamethasone treatment effcts on insulin secretion, molecular and biochemical parameters in submitted to protein restriction

Vanessa Aparecida Gonçalves Giozzet
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 11/02/2008 Português
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A desnutrição e a resistência periférica à insulina induzida por administração de glicocorticóides induzem compensações funcionais e morfológicas em ilhotas pan creáticas a fim de manter a homeostase glicêmica. Deste modo, investigamos as alterações desenvolvidas pelo tratamento com dexametasona (Dex) em animais submetidos à restrição protéica. Foram analisados: parâmetros metabólicos, secreção de insulina em resp osta a glicose e proteínas envolvidas na via de sinalização da insulina em ilhotas pancreáticas isoladas. Ratos submetidos à dieta hipoprotéica (LP) apresentaram características padrões que caracterizam a desnutrição como: diminuição de ganho de massa corp oral, redução dos níveis séricos de albumina, proteína total e insulina. Adicionalmente, os ratos LP exibiram aumento da sensibilidade periférica à insulina e redução da área das ilhotas pancreáticas comparadas ao grupo controle ( P < 0,05). Todos estes parâmetros apresentaram valores similares ao grupo controle nos ratos submetidos à dieta hipoprotéica e submetidos ao tratamento com Dex (LPD), exceto para o peso corpóreo ( P < 0,05). A secreção de insulina em ilhotas pancreáticas isoladas de ratos LPD aprese ntou maior responsividade à glicose...

Imunolocalização e quantificação da proteina SP22 em espermatozoides de ovinos e sua relação com outros parametros espermaticos; Immunolocalization and quantification of the SP22 protein on ovine sperm and its relationship to other sperm parameters

Ana Paula Alves Favareto
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: dissertação de mestrado Formato: application/pdf
Publicado em 21/02/2008 Português
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Vários estudos têm demonstrado que a proteína espermática de membrana SP22 (22-28 kDa) é altamente correlacionada com a fertilidade. A localização desta proteína no segmento equatorial da cabeça espermática em várias espécies mamíferas, e a inibição de fertilização in vivo e in vitro, obtida com a incubação de espermatozóides com anticorpos anti-rSP22, sugerem sua participação na interação espermatozóide-oócito e sua importância para a capacidade reprodutiva masculina. Assim, os objetivos do presente estudo foram avaliar a presença da proteína SP22 em espermatozóides de carneiros adultos (raças Dorper e Santa Inês), identificando sua localização; quantificar os níveis desta proteína mediante as técnicas de ELISA e imunomarcação fluorescente utilizando anticorpo anti-rSP22; e correlacionar os seus níveis com os parâmetros espermáticos de morfologia, integridade de membrana avaliada por sondas fluorescentes (diacetato de carboxifluoresceína e iodeto de propídio), e cinética obtida por sistema computadorizado (CASA). A SP22 foi especificamente localizada no segmento equatorial da cabeça e no colo do espermatozóide ovino. As técnicas de ELISA e imunomarcação fluorescente mostraram-se eficientes para a quantificação da SP22 nos espermatozóides ovinos e altamente correlacionadas (R2 = 0...

Predição de regiões de interface proteina-proteina baseada em informações estruturais; Prediction of protein-protein interface region based on structural information

Roberto Hiroshi Higa
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 30/07/2009 Português
Relevância na Pesquisa
35.77%
Este trabalho aborda o problema de predição de regiões de interface proteína-proteína, baseado em medidas de propriedades físico-químicas e estruturais. A abordagem considera os aminoácidos da superfície da proteína como as unidades básicas para classificação, o que elimina a restrição de uso de patches, considerada por preditores do mesmo tipo. Este preditor é complementado por um segundo preditor, que identifica, dentre os aminoácidos da interface, os mais relevantes do ponto de vista de energia de ligação proteína-proteína, conhecidos como hot spots. A abordagem apresentada permite a utilização dos classificadores de forma independente na predição dos aminoácidos de interface e dos hot spots. Diferente de outras abordagens encontradas na literatura para predição de hot spots, a abordagem empregada não depende do conhecimento da estrutura do complexo protéico e permite que a predição de hot spots seja realizada em complemento à predição dos aminoácidos da interface. O desempenho alcançado pelo preditor na identificação de hot spots é superior ao obtido por outros preditores descritos na literatura e que utilizam o mesmo conjunto de dados e critério de avaliação de desempenho; This work approaches the problem of protein-proteins interface region prediction based on measures of physical-chemical and structural properties. The approach considers the amino acids of the protein surface as the basic units for classification...