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Liver cirrhosis on the colonic anastomotic healing in rats; Cirrose hepática na cicatrização de anastomose intestinal em ratos

BONIFÁCIO, Marcelo di; PARRA, Rogério Serafim; ALMEIDA, Ana Luiza Normanha Ribeiro de; ROCHA, José Joaquim Ribeiro da; FERES, Omar
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.58%
PURPOSE: To investigate the effects of cirrhosis on colonic anastomosis healing in rats. METHODS: Fifty five Wistar male rats were used (23 in the control group and 32 in the cirrhosis group). On the first day of the procedure, the rats in the cirrhosis group underwent double ligation and folding of the common bile duct to induce liver cirrhosis, and the control rats underwent a laparotomy and intestinal manipulation. On the fourteenth and thirty-fifth days, all of the animals were biochemically assessed for serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, bilirubin, total protein, and albumin and for liver histopathology. On the thirty-fifth day, cirrhosis was confirmed. On the twenty-eighth day, all of the animals were subjected to left colon transection and anastomosis. On the seventh day after the colonic anastomosis, the rats were sacrificed and macroscopically evaluated for dehiscence. The region of the colonic anastomosis was removed and subjected to hydroxyproline content measurement, conventional histology, and the immunohistochemical determination of vascular endothelial growth factor (VEGF) and matrix metalloproteinase type 1 (MMP 1). RESULTS: The biochemical and histopathological examinations confirmed cirrhosis in all of the animals in the cirrhosis group. More deaths occurred after anastomosis in the cirrhosis group (5/25) than in the control group (0/21)...

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat

GUERRA, R.R.; TROTTA, M.R.; PARRA, O.M.; AVANZO, J.L.; BATEMAN, A.; ALOIA, T.P.A.; DAGLI, M.L.Z.; HERNANDEZ-BLAZQUEZ, F.J.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66.51%
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-?1 (-53%), TIMP-1 (-31.7%), TGF-?1 (-57.7%), and MMP-2 (-41.6%), whereas Plau mRNA remained unchanged; b) reduced GGT (-43.1%), ALT (-17.6%), and AST (-12.2%) serum levels; c) increased liver weight (11.3%), and reduced liver collagen (-37.1%), regenerative nodules size (-22.1%), and fibrous septum thickness. Progranulin protein (immunohistochemistry) and mRNA (in situ hybridization) were found in fibrous septa and areas of bile duct proliferation in cirrhotic livers. Concluding, HF improved the histology and serum biochemistry of liver cirrhosis...

Efeito da hepatectomia parcial associada à administração de fatores nutricionais hepatotróficos sobre a morfologia, função e expressão de genes pró-fibróticos na cirrose hepática em ratos Wistar induzida por tiocetamida; Effects of partial hepatectomy associated with administration of nutritional hepatotrophic factors in morphology, function and expression of pro-fibrotic genes in thioacetamide-induced liver cirrhosis in Wistar rats

Trotta, Mauricio de Rosa
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: dissertação de mestrado Formato: application/pdf
Publicado em 15/12/2011 Português
Relevância na Pesquisa
56.62%
O presente trabalho avaliou o papel da solução parenteral de fatores hepatotróficos nutricionais em animais com cirrose submetidos à hepatectomia parcial. Este procedimento é temido nestes animais devido à possibilidade de ocorrência de falência hepática aguda, já que a remoção de um fragmento do fígado reduz ainda mais a capacidade funcional de um órgão já comprometido. Além disso, é conhecido que o fígado cirrótico diminui sua capacidade regenerativa, fato que atrasa a recuperação do animal, bem como também regenera cirroticamente. Esses fatores, aliados, contribuem para uma considerável taxa de mortalidade pós-operatória. Porém, há algumas situações em que estes pacientes precisam ser submetidos a ressecções hepáticas, tais como traumas, infecções e neoplasias. De fato, a presença de hepatocarcinomas representa a maior indicação deste procedimento em fígados cirróticos. Por outro lado, tem-se mostrado que a administração parenteral de solução de fatores hepatotróficos nutricionais (FHN), uma mistura de aminoácidos, vitaminas, sais minerais e hormônios, aumenta consideravelmente a proliferação celular e o tamanho do fígado em animais sadios, com fibrose e com cirrose. Nestes dois últimos...

Avaliação morfológica e molecular pós-tratamento com os fatores hepatotróficos, da cirrose  hepática induzida em ratas pela tioacetamida: análise a curto e longo prazo; Morphological and molecular evaluation of liver cirrhosis induced by thioacetamide in rats after treatment with hepatotrophic factors: analysis of short and long term

Silva, Elizangela dos Anjos
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/02/2011 Português
Relevância na Pesquisa
66.54%
A cirrose hepática é um processo irreversível caracterizado pelo desequilíbrio entre a deposição e a degradação de componentes da matriz extracelular (MEC), acarretando disfunção hepatocelular e aumento da resistência ao fluxo sanguíneo, resultando em insuficiência hepática e na hipertensão portal. A cirrose em cães, muitas vezes é identificada nos estágios finais, já que o estágio inicial é assintomático. Em humanos, no estágio final da cirrose, o transplante é o único tratamento. Desta forma, a busca por tratamentos alternativos torna-se imprescindível. A administração de fatores hepatotróficos (FH) tem sido estudada como uma alternativa de tratamento. Este estudo objetivou avaliar os efeitos dos FHs na cirrose hepática murina induzida pela tioacetamida (TAA), imediatamente após o tratamento, e 60 dias pós o seu término. 75 ratas Wistar foram subdivididas em quatro grupos; FH (n=15) e o seu grupo controle (CT, n=30), e FH+60d (n=15) e seu controle (CT, n=15); todos os animais foram induzidos à cirrose hepática pela administração TAA (200mg/Kg), ip, 3 vezes/semana, por 14 semanas. Após a indução, dois grupos receberam a solução de FHs por 12 dias. Um foi eutanasiado imediatamente após o tratamento e o outro...

Análise da expressão de EGFR e de proteínas relacionadas em carcinoma hepatocelular, tecido hepático circunjacente e metástase: estudo clínico-patológico em autópsias; Analysis of the expression of EGFR and related proteins in hepatocellular carcinoma, surrounding liver tissue and metastases : a clinicopathological study in autopsies

Silva, Aloísio Souza Felipe da
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 04/06/2013 Português
Relevância na Pesquisa
56.39%
OBJETIVOS: Analisar a expressão de EGFR, proteínas da via de sinalização ou relacionadas aos seus efeitos em carcinoma hepatocelular (CHC) primários, metastáticos e em tecido hepático não tumoral em autópsias. Correlacionar os achados a dados clínico-patológicos e marcadores de classes moleculares. Avaliar a heterogeneidade de expressão em CHC metastáticos e fatores de disseminação extra-hepática. MÉTODOS: Oitenta autópsias de pacientes com CHC ao exame foram incluídas em estudo retrospectivo transversal. Foram analisados sexo, idade, raça, etilismo, infecção por vírus da hepatite B (VHB) e C (VHC), infecção pelo HIV, tratamento prévio, causas básica e imediata de óbito, peso do fígado, cirrose, número e tamanho dos nódulos, padrão macroscópico, grau histológico, variantes histológicas, padrão arquitetural, invasão de grandes veias e metástases extra-hepáticas. Imuno-histoquímica foi realizada em micromatrizes teciduais para pesquisa de EGFR, pEGFR(Tyr 1173), HER2, ERK1/2, MAPKAPK-2, pMAPK, Ag Ki67, caspase 3, citoqueratina 19 (CK19), mTOR, ciclina D1, Met, vimentina, p53 e beta-catenina. A expressão de EGFR foi avaliada em intensidade (0-3+) e distribuição (0-100%) em um sistema de escores de 0 a 300. Hiperexpressão foi definida para escores >= 200. Amostras de fígado normal foram incluídas como controles. Amostras de CHC primário foram pareadas às suas metástases e consideradas concordantes quando na mesma categoria de expressão. No tecido não tumoral foram semi-quantificadas a reação ductular expressando CK19 e a densidade da população de células estromais perissinusoidais pela vimentina. Estatística foi realizada através dos testes do qui-quadrado ou exato de Fisher ao nível de significância de 0...

Histopatologia do desenvolvimento do modelo de cirrose hepática em ratas pela tioacetamida; Developmental histopathology of the thioacetamide-induced liver cirrhosis in rats

Conrado, André Luiz Veiga
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: dissertação de mestrado Formato: application/pdf
Publicado em 20/02/2014 Português
Relevância na Pesquisa
56.49%
A cirrose é uma doença cosmopolita que causa milhares de mortes a cada ano e encontra-se em estado de atenção pelos sistemas de saúde governamentais. Em auxílio à sua compreensão, utiliza-se o modelo de indução de cirrose pela tioacetamida (TAA) em ratas. Porém este modelo tem carência de informações sobre a presença de células inflamatórias no fígado, as quais desempenham ação destrutiva e estimulam o desenvolvimento da cirrose. Propusemos nesta pesquisa a detecção de macrófagos e células dendríticas neste modelo nas primeiras sete semanas com o uso de tioacetamida na dose de 200mg/kg em aplicações intraperitoneais, correlacionando-as com a apoptose de hepatócitos, a presença de células MCP-1 positivas e o desenvolvimento da fibrose em fígado de ratas. Com isto, esperou-se encontrar o aumento contínuo de macrófagos teciduais e exsudativos, juntamente com o aumento de células dendríticas, atraídas pela secreção de MCP-1 pelas células estreladas hepáticas. Esperou-se também a detecção de hepatócitos em apoptose e consequente deposição de fibras colágenas pelas células estreladas hepáticas. A ação tóxica da tioacetamida foi observada pela necrose de sacabocado e reação tecidual com proliferação de ductos biliares. A tioacetamida foi eficiente em desencadear a presença de macrófagos e hepatócitos em apoptose nas primeiras semanas...

Carcinoma hepatocelular de pequeno tamanho e cirrose hepática pelo vírus da hepatite C: estudo caso-controle de variáveis clínicas e laboratoriais; Small hepatocellular carcinoma and hepatitis C liver cirrhosis. A case-control study based on clinical and laboratorial data

Matielo, Celso Eduardo Lourenço
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: dissertação de mestrado Formato: application/pdf
Publicado em 11/08/2005 Português
Relevância na Pesquisa
66.3%
O carcinoma hepatocelular (CHC) é a quinta neoplasia maligna mais frequente no mundo, sendo que, em mais de 80% dos casos, seu aparecimento está relacionado à presença de cirrose hepática (CH). A infecção crônica pelo vírus da hepatite C (VHC) é uma das principais causas de cirrose hepática no mundo e, consequentemente, de CHC. Este estudo caso-controle foi baseado na análise de variáveis clínicas, bioquímicas e sorológicas de 31 pacientes cirróticos pelo VHC com CHC de pequeno tamanho (; Hepatocellular carcinoma (HCC) is the fifth most common cause of cancer worldwilde. In 80% of cases HCC develop in cirrhotic livers. Hepatitis C virus infection is considered one of the main causes of liver cirrhosis and HCC. We conducted a case-control study involved 31 small HCC cases (

Low bone mineral density in noncholestatic liver cirrhosis: prevalence, severity and prediction

Figueiredo,Fátima Aparecida Ferreira; Brandão,Cynthia; Perez,Renata de Mello; Barbosa,Walnei Fernandes; Kondo,Mario
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2003 Português
Relevância na Pesquisa
56.52%
BACKGROUND: Metabolic bone disease has long been associated with cholestatic disorders. However, data in noncholestatic cirrhosis are relatively scant. AIMS: To determine prevalence and severity of low bone mineral density in noncholestatic cirrhosis and to investigate whether age, gender, etiology, severity of underlying liver disease, and/or laboratory tests are predictive of the diagnosis. PATIENTS/METHODS: Between March and September/1998, 89 patients with noncholestatic cirrhosis and 20 healthy controls were enrolled in a cross-sectional study. All subjects underwent standard laboratory tests and bone densitometry at lumbar spine and femoral neck by dual X-ray absorptiometry. RESULTS: Bone mass was significantly reduced at both sites in patients compared to controls. The prevalence of low bone mineral density in noncholestatic cirrhosis, defined by the World Health Organization criteria, was 78% at lumbar spine and 71% at femoral neck. Bone density significantly decreased with age at both sites, especially in patients older than 50 years. Bone density was significantly lower in post-menopausal women patients compared to pre-menopausal and men at both sites. There was no significant difference in bone mineral density among noncholestatic etiologies. Lumbar spine bone density significantly decreased with the progression of liver dysfunction. No biochemical variable was significantly associated with low bone mineral density. CONCLUSIONS: Low bone mineral density is highly prevalent in patients with noncholestatic cirrhosis. Older patients...

Evaluation of nutritional status of nonhospitalized patients with liver cirrhosis

Carvalho,Luciana; Parise,Edison Roberto
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2006 Português
Relevância na Pesquisa
56.52%
BACKGROUD: Protein-calorie malnutrition is a frequent finding in patients with chronic liver disease, but investigations of nutritional status have been rarely performed in individuals seen at outpatient clinics AIM: To evaluate the nutritional status of patients with alcoholic and nonalcoholic cirrhosis, attended for the first time at a reference outpatient clinic for liver diseases. PATIENTS AND METHODS: A total of 300 consecutive patients attended at the outpatient clinics of a reference center for liver diseases were investigated. Anthropometric evaluation was performed by the usual parameters: triceps skinfold, arm circumference and arm muscle circumference. Biochemical parameters included creatinine/height index, serum albumin and lymphocytes count. The nutritional diagnosis was based on the PCM score proposed by Mendenhall et al. Food intake was retrospectively evaluated using 24-hour dietary recall data. RESULTS: About 71% of the patients studied were chronic alcohol abusers, whereas in 29% cirrhosis was of nonalcoholic etiology. Independently of the disease etiology 75.3% of the patients showed some degree of protein-calorie malnutrition, which was moderate or severe in 38.3% of them. More advanced protein-calorie malnutrition degrees were associated with lower energy and protein intake. The prevalence of moderate or severe protein-calorie malnutrition was higher in patients classified as Child-Pugh C than in patients classified as Child-Pugh A (21% x 58%...

BIOCHEMICAL NUTRITIONAL PROFILE OF LIVER CIRRHOSIS PATIENTS WITH HEPATOCELLULAR CARCINOMA

PORT,Gabriela Zanatta; OLIVEIRA,Kalinca; SOLDERA,Jonathan; TOVO,Cristiane Valle
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2014 Português
Relevância na Pesquisa
66.58%
Context Liver cirrhosis patients with hepatocellular carcinoma present nutritional alterations and metabolic disorders that negatively impact the prognosis. Objective The objective is to identify alterations in the metabolism of macro and micronutrients among liver cirrhosis patients with and without hepatocellular carcinoma and their relation to the Child-Turcote-Pugh score and Barcelona Clinic Liver Cancer staging. Methods Analytical transversal study, with 31 hepatocellular carcinoma patients and 48 liver cirrhosis patients. Laboratorial exams were carried out. The existence of an association between the biochemical parameters and the disease severity as well as the presence of hepatocellular carcinoma was assessed. Results The metabolic-nutritional profile of liver cirrhosis patients caused by the hepatitis C virus and hepatocellular carcinoma showed alterations, specifically the lipid (total cholesterol, HDL and triglycerides), protein (albumin, creatinine and uric acid), iron (transferrin, iron and ferritin saturation), hematocrit and hemoglobin, zinc and B12 vitamin profiles. There is a relation between nutritional biochemical markers and the Child-Turcote-Pugh, as well as Barcelona Clinic Liver Cancer staging. Conclusions Considering the existence of alterations in the metabolism of nutrients in liver cirrhosis patients with and without hepatocellular carcinoma...

TESTOSTERONE CHANGES IN PATIENTS WITH LIVER CIRRHOSIS BEFORE AND AFTER ORTHOTOPIC LIVER TRANSPLANTATION AND ITS CORRELATION WITH MELD

NITSCHE,Rodrigo; COELHO,Júlio Cezar Uili; FREITAS,Alexandre Coutinho Teixeira de; ZENI NETO,Clementino; MARTINS,Eduardo
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE; Colégio Brasileiro de Cirurgia Digestiva - CBCD; Sociedade Brasileira de Motilidade Digestiva - SBMD; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2014 Português
Relevância na Pesquisa
66.44%
Context Hypogonadism is a common clinical situation in male patients with liver cirrhosis. Objectives The aim of the present study was to evaluate the effects of orthotopic liver transplantation on testosterone, free testosterone and sex hormone-binding globulin in male with advanced liver disease and also to determine the relationship of these changes with Model for End-stage Liver Disease (MELD) score. Methods In a prospective study, serum levels of testosterone, free testosterone and sex hormone-binding globulin of 30 male adult patients with end-stage liver disease were measured 2 to 4 hours before and 6 months after orthotopic liver transplantation. Results Total testosterone levels increased after orthotopic liver transplantation and the number of patients with normal testosterone levels increased from 18 to 24. Free testosterone mean level in the pre-transplant group was 7.8 pg/mL and increased to 11.5 pg/mL (P = 0.10) and sex hormone-binding globulin level decreased after orthotopic liver transplantation returning to normal levels in MELD ?18 - group (A) (P

Hypothalamic-pituitary-gonadal function in men with liver cirrhosis before and after liver transplantation

Zacharias,Bruno T.; Coelho,Julio C. U.; Parolin,Mônica B.; Matias,Jorge E. F.; Freitas,Alexandre C. T. de; Godoy,José Luiz de
Fonte: Colégio Brasileiro de Cirurgiões Publicador: Colégio Brasileiro de Cirurgiões
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 Português
Relevância na Pesquisa
66.6%
Objective: To evaluate the influence of end-stage liver disease and orthotopic liver transplantation in the pituitary function and hormone metabolism before and after liver transplantation. Methods: In a prospective study, serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and prolactin (PRL) of 30 male patients with cirrhosis were determined two to four hours before and six months after liver transplantation. The results were compared according to the Model for End-stage Liver Disease (MELD). Results: male patients with liver cirrhosis have hypogonadism. FSH was normal, but inappropriately low due to androgen failure; E2 and PRL, on their turn, were high. After liver transplantation, FSH and LH levels increased (p < 0.05), whereas E2 and PRL normalized (p < 0.05). The MELD score did not influence changes in FSH, PRL and LH, however, the more severe the cirrhosis was, the more significant was the normalization of E2 (p = 0.01). Conclusion: Patients with cirrhosis and male hypogonadism have inappropriately normal levels of FSH and LH, associated with an increase in E2 and LRP. After liver transplantation, FSH and LH increased, while E2 and PRL returned to normal. Changes in E2 levels were most pronounced in patients with MELD > 18. The severity of cirrhosis had no influence on FSH...

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat

Guerra,R.R.; Trotta,M.R.; Parra,O.M.; Avanzo,J.L.; Bateman,A.; Aloia,T.P.A.; Dagli,M.L.Z.; Hernandez-Blazquez,F.J.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2009 Português
Relevância na Pesquisa
66.51%
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-?1 (-53%), TIMP-1 (-31.7%), TGF-?1 (-57.7%), and MMP-2 (-41.6%), whereas Plau mRNA remained unchanged; b) reduced GGT (-43.1%), ALT (-17.6%), and AST (-12.2%) serum levels; c) increased liver weight (11.3%), and reduced liver collagen (-37.1%), regenerative nodules size (-22.1%), and fibrous septum thickness. Progranulin protein (immunohistochemistry) and mRNA (in situ hybridization) were found in fibrous septa and areas of bile duct proliferation in cirrhotic livers. Concluding, HF improved the histology and serum biochemistry of liver cirrhosis...

Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat

Guerra,R.R.; Trotta,M.R.; Parra,O.M.; Avanzo,J.L.; Bateman,A.; Aloia,T.P.A.; Dagli,M.L.Z.; Hernandez-Blazquez,F.J.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2009 Português
Relevância na Pesquisa
66.51%
Nutritional substances associated to some hormones enhance liver regeneration when injected intraperitoneally, being denominated hepatotrophic factors (HF). Here we verified if a solution of HF (glucose, vitamins, salts, amino acids, glucagon, insulin, and triiodothyronine) can revert liver cirrhosis and how some extracellular matrices are affected. Cirrhosis was induced for 14 weeks in 45 female Wistar rats (200 mg) by intraperitoneal injections of thioacetamide (200 mg/kg). Twenty-five rats received intraperitoneal HF twice a day for 10 days (40 mL·kg-1·day-1) and 20 rats received physiological saline. Fifteen rats were used as control. The HF applied to cirrhotic rats significantly: a) reduced the relative mRNA expression of the genes: Col-?1 (-53%), TIMP-1 (-31.7%), TGF-?1 (-57.7%), and MMP-2 (-41.6%), whereas Plau mRNA remained unchanged; b) reduced GGT (-43.1%), ALT (-17.6%), and AST (-12.2%) serum levels; c) increased liver weight (11.3%), and reduced liver collagen (-37.1%), regenerative nodules size (-22.1%), and fibrous septum thickness. Progranulin protein (immunohistochemistry) and mRNA (in situ hybridization) were found in fibrous septa and areas of bile duct proliferation in cirrhotic livers. Concluding, HF improved the histology and serum biochemistry of liver cirrhosis...

Mortality from liver cirrhosis in Espírito Santo State, Brazil

Gonçalves,Patricia Lofego; Gonçalves,Carlos Sandoval; Pereira,Fausto Edmundo Lima
Fonte: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz Publicador: Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
Relevância na Pesquisa
66.5%
To study mortality from liver cirrhosis in Espírito Santo State, Brazil, we reviewed death certificates (DC) from 2000-2010 and medical records of deceased patients with investigation of alcoholism and hepatitis B or C. From a total of 218,410 DC, 3,554 deaths from liver cirrhosis were retrieved. The annual mortality rate was 19.8/100,000 for men and 4.31/100,000 for women, without significant changes after correction for ICD-R98 and R99 and without a significant increase in the annual percentage change. In 49% of death certificates, the aetiology of cirrhosis was defined: of these alcoholism in 81.5% of cases and viral hepatitis in 15.7%. Aetiology was confirmed in 262 reviewed records, including alcoholism (40.5%); hepatitis B or C (26.7%); other (3.8%); and cryptogenic (10.6%). The mean annual potential years of life lost were 5,946 years and 1,739 years for men and women respectively. The mortality rate from cirrhosis in Espírito Santo State is intermediate in relationship to worldwide data; alcoholism and hepatitis B or C were the main aetiologies; probably alcoholism is overestimated and hepatitis B and C viruses are underestimated as causes of cirrhosis registered on death certificates.

Liver cirrhosis on the colonic anastomotic healing in rats

di Bonifácio,Marcelo; Parra,Rogério Serafim; Almeida,Ana Luiza Normanha Ribeiro de; Rocha,José Joaquim Ribeiro da; Feres,Omar
Fonte: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia Publicador: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2011 Português
Relevância na Pesquisa
66.58%
PURPOSE: To investigate the effects of cirrhosis on colonic anastomosis healing in rats. METHODS: Fifty five Wistar male rats were used (23 in the control group and 32 in the cirrhosis group). On the first day of the procedure, the rats in the cirrhosis group underwent double ligation and folding of the common bile duct to induce liver cirrhosis, and the control rats underwent a laparotomy and intestinal manipulation. On the fourteenth and thirty-fifth days, all of the animals were biochemically assessed for serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, bilirubin, total protein, and albumin and for liver histopathology. On the thirty-fifth day, cirrhosis was confirmed. On the twenty-eighth day, all of the animals were subjected to left colon transection and anastomosis. On the seventh day after the colonic anastomosis, the rats were sacrificed and macroscopically evaluated for dehiscence. The region of the colonic anastomosis was removed and subjected to hydroxyproline content measurement, conventional histology, and the immunohistochemical determination of vascular endothelial growth factor (VEGF) and matrix metalloproteinase type 1 (MMP 1). RESULTS: The biochemical and histopathological examinations confirmed cirrhosis in all of the animals in the cirrhosis group. More deaths occurred after anastomosis in the cirrhosis group (5/25) than in the control group (0/21)...

Seroprevalence of hepatitis A immunity among brazilian adult patients with liver cirrhosis: is HAV vaccination necessary?

Oliveira,Luiz Carlos Marques de; Comácio,Samantha Martins; Santos,Júlia de Fátima Gonçalves
Fonte: Brazilian Society of Infectious Diseases Publicador: Brazilian Society of Infectious Diseases
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2011 Português
Relevância na Pesquisa
66.37%
BACKGROUND: Patients with chronic liver disease have a higher risk of fulminant hepatitis when infected with hepatitis A virus, and vaccination of these patients against such infection is recommended. In Brazil, mainly in the South and Southeast regions, the epidemiology of hepatitis A (HA) has shifted from high to intermediate endemicity, which would have implication on policy of HA vaccination for these populations. OBJECTIVE: To verify the prevalence of HA immunity in adult patients with liver cirrhosis (LC), in Uberlândia MG, a city of Southeastern Brazil. METHODS: Between December 2005 and December 2006, 106 patients with LC were consecutively evaluated. In addition, 75 individuals without LC or alcoholism were evaluated (control group - CG). RESULTS: Total anti-HAV (ELISA methods) was positive in 104 (98.1%) patients with LC (82 men, 24 women; mean age, 53.3 ± 11.9 years) and in 74 (98.7%) individuals of the CG (55 men, 20 women; mean age, 47 ± 11.6 years), p > 0.05. CONCLUSIONS: For patients with chronic liver disease, in the geographic regions and age groups evaluated, routine vaccination against hepatitis A is not recommended. Moreover, the serum determination of total anti-HAV, used to assess immunity, is five times cheaper than vaccination against hepatitis A and...

Etiology of liver cirrhosis in Brazil: chronic alcoholism and hepatitis viruses in liver cirrhosis diagnosed in the state of Espfrito Santo

Goncalves,Patricia Lofego; Zago-Gomes,Maria da Penha; Marques,Carla Couzi; Mendonça,Ana Tereza; Gonçalves,Carlos Sandoval; Pereira,Fausto Edmundo Lima
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2013 Português
Relevância na Pesquisa
66.5%
OBJECTIVES: To report the etiology of liver cirrhosis cases diagnosed at the University Hospital in Vitoria, Espirito Santo, Brazil. METHODS: The medical charts of patients with liver cirrhosis who presented to the University Hospital in Vitoria were reviewed. Chronic alcoholism and the presence of hepatitis B or C infections (HBV and HCV, respectively) were pursued in all cases. RESULTS: The sample consisted of 1,516 cases (male:female ratio 3.5:1, aged 53.2±12.6 years). The following main etiological factors were observed: chronic alcoholism alone (39.7%), chronic alcoholism in association with HBV or HCV (16.1 %), HCV alone (14.5%) and in association with alcoholism (8.6%) (total, 23.1 %), and HBV alone (13.1%) and in association with alcoholism (7.5%, total 20.6%). The remaining etiologies included cryptogenic cases (9.8%) and other causes (6.0%). The mean patient age was lower and the male-to-female ratio was higher in the cirrhosis cases that were associated with alcoholism or HBV compared with other causes. Intravenous drug abuse and a history of surgery or blood transfusion were significantly associated with HCV infection. Hepatocellular carcinoma was present at the time of diagnosis in 15.4% of cases. Chronic alcoholism associated with HCV infection was significantly associated (p

Endogenous benzodiazepine-like compounds and diazepam binding inhibitor in serum of patients with liver cirrhosis with and without overt encephalopathy

Avallone, R; Zeneroli, M; Venturini, I; Corsi, L; Schreier, P; Kleinschnitz, M; Ferrarese, C; Farina, F; Baraldi, C; Pecora, N; Frigo, M; Baraldi, M
Tipo: text
Publicado em /06/1998 Português
Relevância na Pesquisa
56.37%
Background/Aim—Despite some controversy, it has been suggested that endogenous benzodiazepine plays a role in the pathogenesis of hepatic encephalopathy. The aim of the present study was to evaluate the concentrations of endogenous benzodiazepines and the peptide, diazepam binding inhibitor, in the blood of patients with liver cirrhosis with and without overt encephalopathy, and to compare these levels with those of consumers of commercial benzodiazepines. ?Subjects—Normal subjects (90), benzodiazepine consumers (14), and cirrhotic patients (113) were studied. ?Methods—Endogenous benzodiazepines were measured by the radioligand binding technique after high performance liquid chromatography (HPLC) purification. The presence of diazepam and N-desmethyldiazepam was assayed by HPLC-electrospray tandem mass spectrometry. Diazepam binding inhibitor was studied in serum by radioimmunoassay. ?Results—Endogenous benzodiazepines were below the limit of detection in 7% of patients with encephalopathy. When detectable, their levels were at least comparable with those of benzodiazepine consumers and correlated with the liver dysfunction but not the stage of encephalopathy. Serum levels of diazepam binding inhibitor tended to decrease when endogenous benzodiazepines levels increased. ?Conclusions—Endogenous benzodiazepines may accumulate in patients with liver cirrhosis during the course of the disease...

A case of variceal bleeding from the jejunum in liver cirrhosis

Park, Chan Woong; Kim, Sae Hee; Yang, Hyeon Woong; Lee, Yun Jung; Jung, Sung Hee; Song, Ho Sup; Lee, Sang Ok; Kim, Anna; Cha, Sang Woo
Fonte: The Korean Association for the Study of the Liver Publicador: The Korean Association for the Study of the Liver
Tipo: text
Português
Relevância na Pesquisa
56.43%
While esophagogastric varices are common manifestations of portal hypertension, variceal bleeding from the jejunum is a rare complication of liver cirrhosis. In addition, ectopic variceal bleeding occurs in the duodenum and at sites of previous bowel surgery in most cases, including of stomas. We report a case of obscure overt gastrointestinal bleeding from jejunal varices in a 55-year-old woman who had not previously undergone abdominal surgery, who had liver cirrhosis induced by the hepatitis C virus. Emergency endoscopy revealed the presence of esophageal varices without stigmata of recent bleeding, and no bleeding focus was found at colonoscopy. She continued to produce recurrent melena with hematochezia and received up to 21 units of packed red blood cells. CT angiography revealed the presence of jejunal varices, but no active bleeding was found. Capsule endoscopy revealed fresh blood in the jejunum. The patient submitted to embolization of the jejunal varices via the portal vein, after which she had a stable hemoglobin level and no recurrence of the melena. This is a case of variceal bleeding from the jejunum in a liver cirrhosis patient without a prior history of abdominal surgery.