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Biologia molecular aplicada às dermatoses tropicais; Molecular biology in tropical dermatoses

ROSELINO, Ana Maria
Fonte: Sociedade Brasileira de Dermatologia Publicador: Sociedade Brasileira de Dermatologia
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
66%
São apresentados conceitos básicos sobre célula, código genético e síntese protéica, e sobre algumas técnicas de biologia molecular, tais como PCR, PCR-RFLP, seqüenciamento de DNA, RT-PCR e immunoblotting. São fornecidos protocolos de extração de nucleotídeos e de proteínas, como salting out no sangue periférico e métodos do fenol-clorofórmio e do trizol em tecidos. Seguem-se exemplos comentados da aplicação de técnicas de biologia molecular para o diagnóstico etiológico e pesquisa em dermatoses tropicais, com ênfase na leishmaniose tegumentar americana e hanseníase.; Initially, basic concepts are presented concerning the cell, genetic code and protein synthesis, and some techniques of molecular biology, such as PCR, PCR-RFLP, DNA sequencing, RT-PCR and immunoblotting. Protocols of nucleotides and of proteins extraction are supplied, such as salting out in peripheral blood allied to phenol-chloroform and trizol methods in skin samples. To proceed, commented examples of application of those techniques of molecular biology for the etiologic diagnosis and for research in tropical dermatoses, with emphasis to American tegumentary leishmaniasis and leprosy are presented.; USP - FAEPA-HCFMRP; CNPq; Fapesp

Molecular biology in tropical dermatoses

ROSELINO, Ana Maria
Fonte: SOC BRASILEIRA DERMATOLOGIA Publicador: SOC BRASILEIRA DERMATOLOGIA
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
65.96%
Initially, basic concepts are presented concerning the cell, genetic code and protein synthesis, and some techniques of molecular biology, such as PCR, PCR-RFLP, DNA sequencing, RT-PCR and immunoblotting. Protocols of nucleotides and of proteins extraction are supplied, such as salting out in peripheral blood allied to phenol-chloroform and trizol methods in skin samples. To proceed, commented examples of application of those techniques of molecular biology for the etiologic diagnosis and for research in tropical dermatoses, with emphasis to American tegumentary leishmaniasis and leprosy are presented.

Estudos sobre dinoflagelados isolados da costa norte do estado de São Paulo: monitoramento, aspectos moleculares, biológicos e químicos; Studies on dinoflagellates from Southern Brazil: monitory, biological and molecular aspects

Naves, Jeanete Lopes
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 22/10/2008 Português
Relevância na Pesquisa
55.88%
Dinoflagelados foram os primeiros microorganismos reconhecidos como fonte primária de certos metabólitos, também conhecidos como ficotoxinas. Em geral são compostos não protéicos, de baixo peso molecular (250-3500 Da), apresentam estruturas químicas e modos de ação bem diferenciados. Envolvidos em florações, mortandade de peixes e intoxicações de seres humanos, devido ao consumo de mariscos contaminados, tornaram alvo de diversas pesquisas. Atividade antifúngica, hemolítica e citotoxicidade contra células tumorais são alguns exemplos da variedade de compostos e modos de ação que podemos encontrar. Como exemplo do potencial de aplicação, a pectenotoxina é candidata a agente quimioterápico e a maitotoxina é um estimulante da síntese e secreção do fator de crescimento. Em alguns casos, estudos com compostos de dinoflagelados alcançam níveis de patente. O risco à saúde pública e as perdas econômicas causadas pelas florações levaram ao estudo do ciclo de vida e interações ecológicas, taxonomia e aspectos moleculares das microalgas envolvidas nesses eventos. Também levou a criação de programas de monitoramento que levam em consideração observações ambientais, amostras de fitoplâncton, mariscos ou peixes...

Um modelo de qualidade para caracterização e seleção de bancos de dados de biologia molecular; A quality model for characterizing and selecting molecular biology databases

Lichtnow, Daniel
Tipo: Tese de Doutorado Formato: application/pdf
Português
Relevância na Pesquisa
66.02%
O número de banco de dados de biologia molecular presentes na Web vem aumentando significativamente nos últimos anos. A dificuldade de localizar estes bancos de dados na Web incentivou a criação de uma série de catálogos. Mesmo com estes catálogos, persiste o desafio de selecionar aqueles bancos de dados que possuem maior qualidade. Normalmente, a seleção é feita por usuários, que nem sempre possuem o conhecimento necessário e enfrentam problemas pela ausência de uma descrição mais rica dos bancos de dados nestes catálogos. Esta ausência de uma descrição mais rica dos bancos de dados gerou iniciativas recentes que visam identificar metadados relevantes para descrição dos bancos de dados de biologia molecular. No entanto, até o momento, como utilizar estes metadados na seleção dos bancos de dados presentes em um catálogo, relacionando estes às dimensões de qualidade de dados, é um tema pouco explorado. Da mesma forma, o uso de Web metrics, utilizadas na seleção de páginas Web, vem sendo quase ignorado na determinação da qualidade de bancos de dados de biologia molecular. Tendo em vista este cenário, nesta tese foi desenvolvido um modelo de qualidade que visa auxiliar na seleção de bancos de dados de biologia molecular presentes em catálogos na Web a partir da avaliação global de um banco de dados por meio de metadados e Web metrics. A definição deste modelo envolve adoção de metadados propostos em outros trabalhos...

Biologia molecular do câncer cervical; Molecular biology of cervical cancer

Rivoire, Waldemar Augusto; Corleta, Helena von Eye; Silva, Ilma Simoni Brum da; Capp, Edison
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
65.91%
A carcinogênese é um processo de múltiplas etapas. Alterações no equilíbrio citogenético ocorrem na transformação do epitélio normal a câncer cervical. Numerosos estudos apoiam a hipótese de que a infecção por HPV está associada com o desenvolvimento de alterações malignas e pré-malignas do trato genital inferior. Neste trabalho são apresentadas as bases para a compreensão da oncogênese cervical. O ciclo celular é controlado por proto-oncogenes e genes supressores. Quando ocorrem mutações, proto-oncogenes tornam-se oncogenes, que são carcinogênicos e causam multiplicação celular excessiva. A perda da ação de genes supressores funcionais pode levar a célula ao crescimento inadequado. O ciclo celular também pode ser alterado pela ação de vírus, entre eles o HPV (Human Papiloma Virus), de especial interesse na oncogênese cervical. Os tipos de HPV 16 e 18 são os de maior interesse, freqüentemente associados a câncer cervical e anal. O conhecimento das bases moleculares que estão envolvidas na oncogênese cervical tem sido possível devido a utilização de técnicas avançadas de biologia molecular. A associação destas técnicas aos métodos diagnósticos clássicos, poderão levar a uma melhor avaliação das neoplasias cervicais e auxiliar no desenvolvimento de novas terapias...

Padrões de comportamento de busca e uso de informação por pesquisadores de biologia molecular e biotecnologia; Behavior standards of information search and use referring to molecular biology and biotechnology

Crespo, Isabel Merlo; Caregnato, Sonia Elisa
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
65.96%
O comportamento de busca e uso de informação dos pesquisadores da área de biologia molecular e biotecnologia foi analisado com base no modelo de David Ellis, verificando como a informação científica em meio digital altera este comportamento. Constituiu-se de pesquisa qualitativa, que, para a coleta de dados, utilizou questões abertas, com entrevista aos pesquisadores do Departamento de Biologia Molecular e Biotecnologia da Universidade Federal do Rio Grande do Sul. Os dados foram trabalhados em uma base de dados desenvolvida no software de análise qualitativa Nvivo versão 2.0, em conformidade com técnicas de codificação o método comparativo constante. Os resultados mostraram um perfil de comportamento de busca e uso de informação do grupo de pesquisadores, com suas principais características, em vários aspectos, geradas pelo uso das tecnologias, destacando a nãolinearidade da busca e obtenção da informação. Constatou-se que os periódicos científicos eletrônicos são a principal fonte de informação destes pesquisadores, e que são amplamente utilizados e aceitos, mas o mesmo não acontece com aqueles de acesso livre, ainda usados de modo restrito. Conclui, também, que o modelo de Ellis é válido, com ampliações e modificações. Sugere novos temas de estudo relacionados ao enfoque pesquisado.; The information seeking and use behavior of Molecular Biology and Biotechnology researchers has been analyzed based on David Ellis´ model...

Update on colorectal carcinomatosis - from molecular biology to diagnosis and management

Martins, Sandra; Longatto Filho, Adhemar
Fonte: SciRes Publicador: SciRes
Tipo: Artigo de Revista Científica
Publicado em //2013 Português
Relevância na Pesquisa
65.91%
After liver metastases, Peritoneal Carcinomatosis (PC) is the second most frequent cause of death in patients with Co-lorectal Cancer (CRC), although the precise incidence of Colorectal Cancer Peritoneal Carcinomatosis is not known, as the majority of the diagnostic studies cannot detect the disease in its initial stages, nowadays, the diagnosis of peritoneal carcinomatosis remains a challenge. The molecular biology of PC is only just beginning to be understood, future knowledge will permit not only identify novel strategies for PC prevention, but also contribute to therapeutic advances, through the development of molecular targeted therapies. The authors performed a literature revision about the Molecu-lar Biology, Diagnosis and Management of Colorectal Cancer Peritoneal carcinomatosis.

Functional markers and a ‘systemic strategy’: convergency between plant breeding, plant nutrition and molecular biology

Arnholdt-Schmitt, Birgit
Fonte: Elsevier SAS Publicador: Elsevier SAS
Tipo: Artigo de Revista Científica Formato: 5885 bytes; application/pdf
Português
Relevância na Pesquisa
55.91%
Research perspectives towards sustainable plant production through plant breeding on abiotic stress tolerance require interdisciplinary competence. The present paper wants to strengthen awareness of important activities or current insights in plant breeding, plant nutrition and molecular biology to facilitate approximation of the disciplines and to direct efforts in molecular biology early in development towards application. As a consequence of recent discussion on appropriate approaches in systems biology towards crop improvement, a global ‘systemic strategy’ is presented to identify functional marker candidates for abiotic stress tolerance more efficiently.

New Strategies on Molecular Biology Applied to Microbial Systematics

HÖFLING,José F.; ROSA,Edvaldo A. R.; BAPTISTA,Mirian J.; SPOLIDÓRIO,Denise M. P.
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/1997 Português
Relevância na Pesquisa
65.91%
Systematics is the study of diversity of the organisms and their relationships comprising classification, nomenclature and identification. The term classification or taxonomy means the arrangement of the organisms in groups (rate) and the nomenclature is the attribution of correct international scientific names to organisms and identification is the inclusion of unknown strains in groups derived from classification. Therefore, classification for a stable nomenclature and a perfect identification are required previously. The beginning of the new bacterial systematics era can be remembered by the introduction and application of new taxonomic concepts and techniques, from the 50?s and 60?s. Important progress were achieved using numerical taxonomy and molecular taxonomy. Molecular taxonomy, brought into effect after the emergence of the Molecular Biology resources, provided knowledge that comprises systematics of bacteria, in which occurs great evolutionary interest, or where is observed the necessity of eliminating any environmental interference. When you study the composition and disposition of nucleotides in certain portions of the genetic material, you study searching their genome, much less susceptible to environmental alterations than proteins...

Molecular biology of the kallikrein-kinin system: from structure to function

Pesquero,J.B.; Bader,M.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/1998 Português
Relevância na Pesquisa
65.85%
The participation of the kallikrein-kinin system, comprising the serine proteases kallikreins, the protein substrates kininogens and the effective peptides kinins, in some pathological processes like hypertension and cardiovascular diseases is still a matter of controversy. The use of different experimental set-ups in concert with the development of potent and specific inhibitors and antagonists for the system has highlighted its importance but the results still lack conclusivity. Over the last few years, transgenic and gene-targeting technologies associated with molecular biology tools have provided specific information about the elusive role of the kallikrein-kinin system in the control of blood pressure and electrolyte homeostasis. cDNA and genomic sequences for kinin receptors B2 and B1 from different species were isolated and shown to encode G-protein-coupled receptors and the structure and pharmacology of the receptors were characterized. Transgenic animals expressing an overactive kallikrein-kinin system were established to study the cardiovascular effects of these alterations and the results of these investigations further corroborate the importance of this system in the maintenance of normal blood pressure. Knockout animals for B2 and B1 receptors are available and their analysis also points to the role of these receptors in cardiovascular regulation and inflammatory processes. In this paper the most recent and relevant genetic animal models developed for the study of the kallikrein-kinin system are reviewed...

Molecular biology and clinical implication of hepatitis C virus

Lyra,A.C.; Fan,X.; Di Bisceglie,A.M.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/05/2004 Português
Relevância na Pesquisa
65.85%
Hepatitis C virus (HCV) was first described in 1989 as the putative viral agent of non-A non-B hepatitis. It is a member of the Flaviviridae family and has been recognized as the major causative agent of chronic liver disease, including chronic active hepatitis, cirrhosis and hepatocellular carcinoma. HCV is a positive RNA virus with a genome containing approximately 9500 nucleotides. It has an open reading frame that encodes a large polyprotein of about 3000 amino acids and is characterized by extensive genetic diversity. HCV has been classified into at least 6 major genotypes with many subtypes and circulates within an infected individual as a number of closely related but distinct variants known as quasispecies. This article reviews aspects of the molecular biology of HCV and their clinical implication.

Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET)

Wiebe,Leonard I.
Fonte: Instituto de Tecnologia do Paraná - Tecpar Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/05/2007 Português
Relevância na Pesquisa
56.03%
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology. Originally, molecular biology meant the biology of gene expression, but now molecular biology broadly encompasses the macromolecular biology and biochemistry of proteins, complex carbohydrates and nucleic acids. To date, molecular imaging has focused primarily on proteins, with emphasis on monoclonal antibodies and their derivative forms, small-molecule enzyme substrates and components of cell membranes, including transporters and transmembrane signalling elements. This overview provides an introduction to nucleosides...

Teaching Cell and Molecular Biology for Gender Equity

Sible, Jill C.; Wilhelm, Dayna E.; Lederman, Muriel
Fonte: American Society for Cell Biology Publicador: American Society for Cell Biology
Tipo: text
Publicado em //2006 Português
Relevância na Pesquisa
55.88%
Science, technology, engineering, and math (STEM) fields, including cell biology, are characterized by the “leaky pipeline” syndrome in which, over time, women leave the discipline. The pipeline itself and the pond into which it empties may not be neutral. Explicating invisible norms, attitudes, and practices by integrating social studies of science into science education may be the necessary first step in helping female students persist in STEM disciplines. In 2003 and 2004, a sophomore Cell and Molecular Biology course at Virginia Tech (Blacksburg, VA) was taught integrating social studies of science with standard material. The course was successfully implemented, teaching students factual content while increasing awareness of the cultures of science and their self-confidence in engaging with the subject. Course evaluation data indicated that females in particular perceived greater gains in logical thinking and problem-solving abilities than females in a traditional cell biology course. Consistent with K–12 studies, males in this class were likely to view scientists as male only, whereas females viewed scientists as male and female. This pilot project demonstrates that social studies can be integrated successfully in a cell biology course. Longitudinal studies of this cohort of students will indicate whether this approach contributes to the retention of women in the field.

Founding, early history, and transformation of the Journal for Lipid Research to an American Society of Biochemistry and Molecular Biology journal1

Dennis, Edward A.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: text
Publicado em /04/2009 Português
Relevância na Pesquisa
65.85%
The Journal of Lipid Research was founded in October, 1959 and has had a long and distinguished history. It evolved from an initial concept of a loose-leaf methodology handbook to a major journal for the lipid field. Its growth has in many ways paralleled the growth and expansion of lipid research. Today, it is operated as a journal of the American Society for Biochemistry and Molecular Biology at the forefront of biomedical research on lipids.

From Genes to Proteins to Behavior: A Laboratory Project That Enhances Student Understanding in Cell and Molecular Biology

Aronson, Benjamin D.; Silveira, Linda A.
Fonte: American Society for Cell Biology Publicador: American Society for Cell Biology
Tipo: text
Publicado em //2009 Português
Relevância na Pesquisa
55.91%
In the laboratory, students can actively explore concepts and experience the nature of scientific research. We have devised a 5-wk laboratory project in our introductory college biology course whose aim was to improve understanding in five major concepts that are central to basic cellular, molecular biology, and genetics while teaching molecular biology techniques. The project was focused on the production of adenine in Saccharomyces cerevisiae and investigated the nature of mutant red colonies of this yeast. Students created red mutants from a wild-type strain, amplified the two genes capable of giving rise to the red phenotype, and then analyzed the nucleotide sequences. A quiz assessing student understanding in the five areas was given at the start and the end of the course. Analysis of the quiz showed significant improvement in each of the areas. These areas were taught in the laboratory and the classroom; therefore, students were surveyed to determine whether the laboratory played a role in their improved understanding of the five areas. Student survey data demonstrated that the laboratory did have an important role in their learning of the concepts. This project simulated steps in a research project and could be adapted for an advanced course in genetics.

Mathematical Biology Modules Based on Modern Molecular Biology and Modern Discrete Mathematics

Robeva, Raina; Davies, Robin; Hodge, Terrell; Enyedi, Alexander
Fonte: American Society for Cell Biology Publicador: American Society for Cell Biology
Tipo: text
Publicado em //2010 Português
Relevância na Pesquisa
55.88%
We describe an ongoing collaborative curriculum materials development project between Sweet Briar College and Western Michigan University, with support from the National Science Foundation. We present a collection of modules under development that can be used in existing mathematics and biology courses, and we address a critical national need to introduce students to mathematical methods beyond the interface of biology with calculus. Based on ongoing research, and designed to use the project-based-learning approach, the modules highlight applications of modern discrete mathematics and algebraic statistics to pressing problems in molecular biology. For the majority of projects, calculus is not a required prerequisite and, due to the modest amount of mathematical background needed for some of the modules, the materials can be used for an early introduction to mathematical modeling. At the same time, most modules are connected with topics in linear and abstract algebra, algebraic geometry, and probability, and they can be used as meaningful applied introductions into the relevant advanced-level mathematics courses. Open-source software is used to facilitate the relevant computations. As a detailed example, we outline a module that focuses on Boolean models of the lac operon network.

The Role of Molecular Biology in the Biomonitoring of Human Exposure to Chemicals

Muñoz, Balam; Albores, Arnulfo
Fonte: Molecular Diversity Preservation International (MDPI) Publicador: Molecular Diversity Preservation International (MDPI)
Tipo: text
Publicado em 12/11/2010 Português
Relevância na Pesquisa
55.93%
Exposure to different substances in an occupational environment is of utmost concern to global agencies such as the World Health Organization and the International Labour Organization. Interest in improving work health conditions, particularly of those employees exposed to noxious chemicals, has increased considerably and has stimulated the search for new, more specific and selective tests. Recently, the field of molecular biology has been indicated as an alternative technique for monitoring personnel while evaluating work-related pathologies. Originally, occupational exposure to environmental toxicants was assessed using biochemical techniques to determine the presence of higher concentrations of toxic compounds in blood, urine, or other fluids or tissues; results were used to evaluate potential health risk. However, this approach only estimates the presence of a noxious chemical and its effects, but does not prevent or diminish the risk. Molecular biology methods have become very useful in occupational medicine to provide more accurate and opportune diagnostics. In this review, we discuss the role of the following common techniques: (1) Use of cell cultures; (2) evaluation of gene expression; (3) the “omic” sciences (genomics...

Implementation of a Project-Based Molecular Biology Laboratory Emphasizing Protein Structure–Function Relationships in a Large Introductory Biology Laboratory Course

Treacy, Daniel J.; Sankaran, Saumya M.; Gordon-Messer, Susannah; Saly, Danielle; Miller, Rebecca; Isaac, Stefan R.; Kosinski-Collins, Melissa S.
Fonte: American Society for Cell Biology Publicador: American Society for Cell Biology
Tipo: text
Publicado em 01/03/2011 Português
Relevância na Pesquisa
55.88%
In introductory laboratory courses, many universities are turning from traditional laboratories with predictable outcomes to inquiry-inspired, project-based laboratory curricula. In these labs, students are allowed to design at least some portion of their own experiment and interpret new, undiscovered data. We have redesigned the introductory biology laboratory course at Brandeis University into a semester-long project-based laboratory that emphasizes concepts and contains an element of scientific inquiry. In this laboratory, students perform a site-directed mutagenesis experiment on the gene encoding human ?D crystallin, a human eye lens protein implicated in cataracts, and assess the stability of their newly created protein with respect to wild-type crystallin. This laboratory utilizes basic techniques in molecular biology to emphasize the importance of connections between DNA and protein. This project lab has helped engage students in their own learning, has improved students’ skills in critical thinking and analysis, and has promoted interest in basic research in biology.

The Molecular Biology Capstone Assessment: A Concept Assessment for Upper-Division Molecular Biology Students

Couch, Brian A.; Wood, William B.; Knight, Jennifer K.
Fonte: American Society for Cell Biology Publicador: American Society for Cell Biology
Tipo: text
Publicado em 02/03/2015 Português
Relevância na Pesquisa
55.88%
This article describes the development of the Molecular Biology Capstone Assessment, a multiple-true/false concept assessment targeted to upper-division students. This assessment is intended to help pinpoint areas of conceptual difficulty among graduating majors in order to facilitate curricular change at the departmental level.

7.013 Introductory Biology, Spring 2005; Introductory Biology

Sive, Hazel L.; Jacks, Tyler; Gardel, Claudette L.
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Português
Relevância na Pesquisa
56.04%
The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material. 7.014 focuses on the application of the fundamental principles toward an understanding of human biology. Topics include genetics, cell biology, molecular biology, disease (infectious agents, inherited diseases and cancer), developmental biology, neurobiology and evolution.